Effects of increasing dietary arginine supply during the three first weeks after weaning on pig growth performance, plasma amino acid concentrations, and health status.

A total of 425 weaned pigs (Exp. 1: 225 pigs [5.8 ±â€…0.9 kg]; Exp. 2: 200 pigs [6.1 ±â€…1.2 kg]) were used to determine the optimal dietary standardized ileal digestible (SID) arginine (Arg) level in early nursery diets based on growth and health responses. The basal diet in Exp.1 was formulated to meet SID Arg recommendation (0.66%; NRC, 2012) and in Exp. 2, SID Arg was set to simulate current industry practices for feeding nursery pigs (1.15 %). Basal diets were supplemented with 0.3%, 0.6%, 0.9%, and 1.2% of l-arginine to provide five levels of dietary SID Arg. Experimental diets were fed during phases I (days 0 to 7) and II (days 8 to 21) with common diets until market. Feed disappearance and body weight (BW) were measured on days 7, 14, 21, and 43. Final BW was recorded at first removal of pigs for market. Pen fecal score was assigned daily from days 0 to 21. Plasma immunoglobulin A (IgA) was determined on days 0, 7, and 14 and amino acids (AAs) concentration and plasma urea nitrogen (PUN) on days 0 and 14. Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of dietary Arg. Optimal SID Arg was determined by fitting the data with piecewise regression, using growth performance as the primary response variable. In Exp. 1, dietary Arg linearly increased (P < 0.1) BW, average daily gain (ADG), and gain to feed ratio (G:F) ratio on day 21, as well as reduced (χ2 = 0.004) the percentage of pigs that lost weight (PLW) in week 1 by 29%. Dietary Arg resulted in linear improvement (P = 0.082) of ADG for the overall nursery period and quadratic improvement (P < 0.1) of final BW at marketing. In Exp. 2, dietary Arg linearly increased (P < 0.05) ADG and average daily feed intake (ADFI) in week 1, BW and ADFI (P < 0.1) on day 14, as well as reduced (χ2 ≤ 0.001) PLW in week 1. From days 0 to 21, G:F was improved quadratically (P < 0.1). Dietary Arg linearly increased (P < 0.1) ADG and BW on day 43. Dietary Arg supplementation decreased the incidence (χ2 < 0.05) of soft and watery feces during the first weeks after weaning and lower concentration of plasma IgA on days 7 and 14. Dietary Arg linearly and/or quadratically influenced plasma AA concentrations (P < 0.05), including an increase in Arg, Leu, Phe, Val, citrulline, ornithine, and PUN concentrations. Overall, weaned pigs exhibit optimal nursery growth performance and health when provided with dietary SID Arg ranging from 1.5% to 1.9%. This dietary range contributes to a reduction in the occurrence of fall-back pigs and improvements in final BW at marketing.

Nutrient Intake, Performance, and Body Composition of Preseason Wrestlers.

College athletes, especially in weight class sports, often experience energy deficits. Athletes competing in weight class sports such as wrestling are at greatest risk for deficiencies and little is known about the relationships between body composition, nutrient intake, and performance in these athletes. The purposes of this study were to (1) quantify macronutrient and micronutrient intake of pre-season male collegiate wrestlers and compare to estimated needs, and (2) examine relationships among nutritional intakes, body composition, and performance measurements of strength and anaerobic capacity. Male Division I wrestlers (n = 11, age: 21.3 ± 1.7 years, wrestling experience: 14.9 ± 2.5 years) were recruited during pre-season. Nutrient intake was collected from a 3-day food diary. A 7-site skinfold assessment determined fat-free mass (FFM) to estimate total daily energy expenditure (eTEE). Isokinetic and isometric strength were evaluated by a Biodex dynamometer. Anaerobic capacity was tested on a stationary cycle ergometer. Eight of eleven wrestlers were energy deficient based on estimated needs. Mean intake of four micronutrients fell below the Recommended Dietary Allowances (RDA). Significant correlations were found between dietary intake, strength and anaerobic performance variables (r = 0.603 - 0.902, p = 0.0001 - 0.05). However, after accounting for FFM, these relationships were no longer significant. Nutrient intake in tandem with body composition affects performance for weight class athletes. Achieving high FFM during the preseason may be advantageous for wrestling performance. Nutrient intake and body composition should be monitored so coaches and health professionals can create individualized recommendations to help athletes optimize performance.

Effects of crude protease produced by Bacillus subtilis (MAFIC Y7) on growth performance, immune indices, and anti-inflammatory responses of broilers fed soybean meal- or cottonseed meal-based diets.

A strain of Bacillus subtilis (MAFIC Y7) was isolated from the intestine of Tibetan pigs and was able to express high protease activity. The aim of this study was to characterize the proteases produced by MAFIC Y7, and to investigate the effects of protease addition on growth performance, ileal amino acid digestibility, and serum immunoglobulin and immune factors of broilers fed SBM-based diets, or on growth performance, carcass characteristics, and intestinal morphology of broilers fed CSM-based diets. B. subtilis (MAFIC Y7) expressed protease showed its optimal enzyme activity at 50 °C and pH 7.0. The coated crude enzyme (CCE) showed greater stability at pH 3.0 than its uncoated counterpart. Experiment 1 was conducted with six diets based on three levels of crude protein (CP)-CPlow, CPmedium, and CPhigh-with or without CCE. In CPlow, CCE increased gain:feed (G:F) (days 1 to 21, days 1 to 42) by 8%, 3%, respectively, and enhanced apparent ileal digestibility (AID) of crude protein and lysine (on day 42) by 8.8%, 4.6%, respectively, compared with diets containing no CCE (P < 0.05). CCE increased G:F from days 1 to 21 from 0.63 to 0.68, improved G:F and average daily gain (ADG) during days 1 to 42, and enhanced AID of crude protein, lysine, cysteine, and isoleucine on day 42 compared with the unsupplemented treatments (in CPmedium, P < 0.05). CCE increased serum IgA (on day 21), serum IgA and IgG and increased serum IL-10 (on day 42), but decreased serum tumor necrosis factor-α (TNF-α; on day 21), and serum IL-8 and TNF-α (on day 42) compared with unsupplemented treatments. At CPhigh, CCE decreased serum levels of IL-6 and TNF-α (on day 21), and IL-8 and TNF-α (on day 42) compared with unsupplemented treatments (in CPhigh, P < 0.05). In experiment 2, CSM-based diets with two lysine-to-protein ratios (5.2% or 5.5%) with or without CCE. In the high Lys diet (5.5% Lys:protein), CCE increased ADG and G:F, increased carcass, but decreased abdominal fat compared with the unsupplemented treatment (P < 0.05). In the 5.2% Lys:protein dietary treatment, CCE improved duodenal villus height compared with the unsupplemented treatment (P < 0.05). Supplementation of protease produced by MAFIC Y7 was associated with lower inflammatory responses in SBM diets (CPmedium or CPhigh) and improved ADG in broilers fed CPmedium or CPhigh. The proteases improved ADG and the efficiency of CSM use when the ratio of Lys to protein was 5.5%.

The aim of this study was to investigate the effects of Bacillus subtilis (MAFIC Y7)-expressed protease on reducing inflammatory responses of soybean meal (SBM) diets and improving the efficiency of cottonseed meal (CSM) in broilers. Experiment 1 was conducted with six dietary treatments based on three levels of crude protein (CP)­CPlow, CPmedium, and CPhigh­without or with proteases (0 or 4,000 U/kg). Supplementation of proteases significantly improved growth performance, gain:feed (G:F), and apparent ileal digestibility of crude protein and amino acids (cysteine, isoleucine, and histidine) in broilers fed CPmedium treatment (P < 0.05). Proteases inhibited inflammatory responses in SBM-based diets by decreasing serum tumor necrosis factor-α (TNF-α) (in CPmedium and CPhigh), and interleukin (IL)-6 (in CPhigh); and IL-8 and TNF-α (in CPmedium and CPhigh) on day 21. In experiment 2, broilers were fed with CSM-based diets with two ratios of lysine-to-protein (5.2% or 5.5%) with or without proteases. Proteases in the diet of 5.5% Lys to protein ratio increased growth performance and G:F compared to diets without proteases (P < 0.05). Proteases produced by MAFIC Y7 improved growth performance and G:F in CPmedium. Supplementation of protease was associated with lower inflammatory responses of broilers fed SBM-based diets (CPmedium or CPhigh) and improved the efficiency of CSM use when the ratio of lysine-to-protein was 5.5%.

A corn-fermented protein ingredient can be included in early nursey diets without compromising pig growth performance and health status.

In nursery diets, ingredients with high protein content and highly digestible nutrients, such as corn-fermented protein product with added yeast mass (GDDY), can be included as an alternative to common protein sources. This study investigated the dietary inclusion of GDDY as an alternative protein source on growth performance and intestinal health of weaned pigs. A total of 594 weaned pigs (5.7 ±â€…0.9 kg; 18.5 days of age) were allotted to 36 pens in a randomized incomplete block design. Pens were assigned to one of 4 dietary treatments: CON: a common nursery feeding program; SBM75: CON diet replacing 75% of soybean meal (SBM) with GDDY; FM/ESBM: CON diet without fish meal (FM) and enzyme-treated SBM (ESBM) + GDDY; GDDY50: CON diet replacing 50% of SBM, FM, and ESBM with GDDY. Experimental diets were formulated to meet nutrient requirements of nursery pigs and provided in meal form through four phases during the nursery period. Pig growth performance was assessed on days 7, 14, 21, 28, 42, and 53. Pen fecal score was assessed daily from days 0 to 14, and 3 times per week from days 15 to 35. Intestinal health was assessed based on plasma immunoglobulin A (IgA) concentration and the differential sugar absorption test. The total tract digestibility of dry matter (DM), crude protein (CP), gross energy (GE), and phosphorus was also evaluated. From days 0 to 7 and days 7 to 14, dietary treatment had no effect (P > 0.05) on BW, ADG, and ADFI. For the rest of the experimental period, ADG and ADFI were greater (P < 0.05) in pigs fed CON in comparison with those fed SBM75 and GDDY50 and did not differ from pigs fed FM/ESBM. Pigs fed GDDY50 tended (P = 0.082) to have greater serum IgA concentration on day 20 when compared with SBM75 and FM/ESBM pigs. There were no differences among dietary treatments for DM, CP, and GE digestibility. Phosphorus digestibility was higher in FM/ESBM (P < 0.05) compared with SBM75 and GDDY50. These results supported the hypothesis that GDDY can be incorporated in nursery pig diets during the first couple weeks after weaning without affecting growth performance. However, in the late nursery period, inclusion levels starting at 14% can compromise performance.

Evaluation of multienzyme supplementation and fiber levels on nutrient and energy digestibility of diets fed to gestating sows and growing pigs.

The objective was to investigate the effect of a multienzyme blend (MEblend) and inclusion level on apparent total tract digestibility (ATTD) of energy and nutrients, as well as ileal digestibility of crude protein (CP) and amino acids (AA) in gestation diets with low (LF) or high-dietary fiber (HF) fed to gestation sows. For comparison, growing pigs were fed the same HF diets to directly compare ATTD values with the gestating sows. In experiment 1, 45 gestating sows (parity 0 to 5; 187 ±â€…28 kg bodyweight; BW) were blocked by parity in a 2 × 3 factorial arrangement and fed 2.2 kg/d of the HF (17.5% neutral detergent fiber; NDF) or LF (13% NDF) diet and one of three levels of MEblend (0.0%, 0.08%, and 0.1%) to determine impacts of MEblend on ATTD. Twenty-seven growing pigs (initial 35.7 ±â€…3.32 kg BW) were fed the same HF diet (5% of BW) and one of three MEblend inclusions. The MEblend at both 0.08% and 0.1% increased ATTD of energy, NDF, and acid detergent fiber (ADF) (P < 0.05) in gestating sows but ATTD of total non-starch polysaccharides (NSP) and its residues were not affected. Sows fed HF, regardless of MEblend, had greater ATTD of NDF, xylose, and total NSP (P < 0.05) in comparison to grower pigs. In experiment 2, ileal cannulas were placed in 12 gestating sows (parity 0 to 2; BW 159 ±â€…12 kg) to determine apparent and standardized ileal digestibility (AID and SID) of AA and NSP. In a crossover design, sows were fed the same six diets, as in experiment 1, and a nitrogen-free diet during five periods of seven days each to achieve eight replicates per diet. There was no interaction between diet fiber level and MEblend inclusion. Supplementation of MEblend to gestating sow diets did not impact SID of CP and AA regardless of dietary fiber level. The SID of His, Ile, Lys, Phe, Thr, Trp, and Val were 3% to 6% lower (P < 0.09) in HF than LF independent of MEblend. Supplementation of MEblend did not impact AID of NSP components, but sows fed HF had higher AID of arabinose (LF: 26.5% vs. HF: 40.6%), xylose (LF: 3.5% vs. HF: 40.9%), and total NSP (LF: 25.9% vs. HF: 40.0%) compared to sows fed LF (P < 0.05). Dietary supplementation of MEblend increased ATTD of nutrients, NSP, and energy in diets fed to gestating sows regardless of inclusion level, with MEblend having a greater incremental increase in diets with lower NDF levels. Inclusion of MEblend impacted neither SID of AA nor AID of NSP in low- or high-fiber gestation diets, but high-fiber diet, negatively affected SID of AA.

Fiber-degrading enzymes have been extensively studied in growing pigs with minimal studies focusing on gestating sows; however, commercial gestating sow diets often contain more fiber than grower pig diets to stimulate the sensation of satiety without influencing weight gain. A challenge with dietary fiber is its hindrance on digestibility of nutrients. Supplementation of multienzyme blends increases nutrient digestibility of fibrous diets in grower pigs, but there is little data characterizing the effects of fiber-degrading enzymes in gestation diets for pregnant sows. In this study, inclusion of a multienzyme comprised of various carbohydrases and a protease in gestation diets increased apparent total tract digestibility of nutrients and energy for both gestating sows and growing pigs; however, digestibility of non-starch polysaccharides was only improved in growing pigs. Enzyme supplementation to gestating sow diets had limited impact on the ileal digestibility of nutrients, but ileal digestibility of amino acids and crude protein was reduced when gestating sows were fed diets higher in neutral detergent fiber. When formulating high-fiber diets for gestating sows and growing pigs using similar ingredients, it is critical to consider the differences in digestibility of fibrous components, particularly regarding ileal digestibility of amino acids.

The Linear-Logistic Model: A Novel Paradigm for Estimating Dietary Amino Acid Requirements.

This study aimed to determine whether current methods for estimating AA requirements for animal health and welfare are sufficient. An exploratory data analysis (EDA) was conducted, which involved a review of assumptions underlying AA requirements research, a data mining approach to identify animal responses to dietary AA levels exceeding those for maximum protein retention, and a literature review to assess the physiological relevance of the linear-logistic model developed through the data mining approach. The results showed that AA dietary levels above those for maximum growth resulted in improvements in key physiological responses, and the linear-logistic model depicted the AA level at which growth and protein retention rates were maximized, along with key metabolic functions related to milk yield, litter size, immune response, intestinal permeability, and plasma AA concentrations. The results suggest that current methods based solely on growth and protein retention measurements are insufficient for optimizing key physiological responses associated with health, survival, and reproduction. The linear-logistic model could be used to estimate AA doses that optimize these responses and, potentially, survival rates.

Nutritional value of high protein ingredients fed to growing pigs in comparison to commonly used protein sources in swine diets.

Two experiments were conducted to test the hypothesis that two high protein dried distillers with solubles and yeast mass added (GDDY) products fed to growing pigs had comparable amino acid (AA) digestibility and metabolizable energy (ME) to feeds commonly used in swine diets. In experiment 1, seven barrows with an initial body weight (BW) of 25 ±â€…0.8 kg were fitted with a simple T-cannula at the distal ileum to allow for digesta collection. Experimental diets were N-free diets and six cornstarch-based diets containing six ingredients as the sole source of AA: spray dried GDDY, ring dried GDDY, corn distillers dried grains with solubles (DDGS), soybean meal (SBM), enzymatically treated soybean meal (ESBM), and fish meal (FM) provided at 4% of BW. The experiment was conducted as a 7 × 7 Latin square design with seven collection periods of 7 d (5 d adaptation and 2 d ileal digesta collection). In experiment 2, a total of 28 barrows (28.8 ±â€…1.4 kg BW) were used in a two-period switch-back design with seven diets and four replicate pigs in each period (n = 8 reps per diet). Experimental diets were a corn-based basal diet and six corn-based diets containing spray dried GDDY, ring dried GDDY, DDGS, SBM, ESBM, and FM. Fecal and urine samples were collected using the marker-to-marker approach for 5 d after 7 d of adaptation to determine ME concentration. Overall, standardized ileal digestibility (SID) values were within the mean ±â€…SD of NRC (2012) values for all ingredients evaluated. The SID of AA was greater (P < 0.05) in ESBM than the other protein feedstuffs (90.09% vs. 78.71%-81.51%). There were no significant differences in SID of AA (P > 0.05) in SBM, FM, spray dried GDDY, and ring dried GDDY (81.49%, 78.71%, 81.52%, and 79.20%). With respect to the most common first limiting AA for swine, the SID of Lys was greater (P < 0.05) in spray dried GDDY than ring dried GDDY and DDGS (83.56% vs. 77.33% and 68.53%, respectively). There were no significant differences (P > 0.05) for ME in corn (3,313 kcal/kg), ESBM (3,323 kcal/kg), and FM (3,454 kcal/kg) when compared with spray dried GDDY and ring dried GDDY (3,995 and 3,442 kcal/kg respectively). However, spray dried GDDY had greater DE and ME when compared to SBM. Collectively, this study demonstrates that GDDY products have an AA profile and digestibility comparable to SBM; ME in GDDY products is not different from corn. Therefore, GDDY has the potential as a feed ingredient for pigs, which could provide an alternative source of protein and energy in swine diets.

Co-products such as distiller's dried grains with solubles (DDGS) from the ethanol industry are obtained through fermentation processes and removal of nonfermentable components; separation of soluble fiber and protein components can potentially increase their nutritional value in swine diets. With the development of new feed processing technologies, high protein DDGS with added yeast mass (GDDY) has been obtained by separating the protein fraction of DDGS. However, there is limited information on the nutritional value of these co-products for pigs. The first step in determining the suitability of novel co-product use in swine diets is to define the nutritional value. Therefore, the nutritional value of GDDY was determined by analyzing standardized ileal digestibility (SID) of amino acids (AA) and metabolizable energy (ME) in growing pigs compared with common protein and energy feeds used in growing pig diets. The results show that the AA profile and digestibility in GDDY products were not different from soybean meal (SBM) as is the case for lysine, the first limiting AA in pig diets. Similarly, ME concentration in GDDY does not differ from those in other energy sources. Therefore, GDDY could be an alternative source of protein and energy in swine diets, and the obtained data allow its inclusion in pig diets formulation.

Evaluation of standardized ileal digestibility of amino acids and metabolic availability of methionine, using the indicator amino acid oxidation method, in black soldier fly larvae (Hermetia illucens) meal fed to growing pigs.

Standardized ileal digestibility (SID, %) of crude protein (CP) and amino acid (AA) and the metabolic availability (MA) of Met using the indicator amino acid oxidation (IAAO) method, in partially defatted black soldier fly larvae (PD-BSFL) meal were determined in growing pigs in 2 experiments. The Met SID value was then compared numerically with the Met MA to understand how different SID is compared with its MA value. In Exp. 1, 6 ileal-cannulated barrows (initial body weight [BW] = 18.03 ± 0.34 kg) were used in a 2-period switch back design and fed either a nitrogen-free diet (NFD) or test diet, with PD-BSFL meal as the sole source of AA, over two 11-d experimental periods, at a feeding level of 2.8 × estimated maintenance digestible energy requirement. Barrows were adapted for 9-d to the diet, followed by continuous 8-h ileal digesta collection on day 10 and 11. Digesta were pooled per pig within period. The SID of CP and Met of PD-BSFL meal were 76.1 ± 6.2% and 90.4 ± 3.9%, respectively. In Exp. 2, 7 barrows (initial BW = 18.77 ± 0.69 kg) were used in a 7 × 7 Latin square design with L-[1-13C]-Phe as the indicator AA. Each pig was randomly assigned to 1 of 7 dietary treatments over seven 3-d experimental periods. Two diet types were studied including reference (crystalline AA) and PD-BSFL test diets, each supplying graded intakes of Met at 55, 65, and 75% of the estimated SID requirement (NRC, 2012). The MA of Met was determined by comparing the IAAO response between the reference and PD-BSFL test diet using the slope-ratio method. Linear regression determined a negative slope of the best fit line for both the reference and test diets (P < 0.05). The MA of Met in PD-BSFL meal was 53.3%, which is as expected lower than the SID value. While it is generally appreciated that MA will be less than SID, the use of SID is more practical. In cases where SID cannot explain physiological outcomes of feeding a novel ingredient, IAAO may provide additional insight into whether MA should be explored.

The interest in black soldier fly larvae (BSFL) meal as a protein ingredient in swine feed has grown in the past years. As a novel protein ingredient, it is beneficial to evaluate the amino acid (AA) digestibility and metabolic availability (MA) of the limiting AA, Met, in pigs, in BSFL meal prior to incorporation in feed for a more precise formulation. Two different methodologies were used to determine the AA digestibility and MA, standardized ileal digestibility (SID) and indicator amino acid oxidation (IAAO) method, respectively. Based on the SID values, the AA digestibility of BSFL meal, for some, but not all AA, is comparable to other commonly used protein ingredients in commercial swine feed. When compared with the MA result of Met, the Met SID value is much lower. This indicates that not all digested Met is available for protein synthesis or other metabolic processes in the animal.

Dietary Lactobacillus reuteri prevent from inflammation mediated apoptosis of liver via improving intestinal microbiota and bile acid metabolism.

Lactobacillus reuteri (L. reuteri) has been recognized as a probiotic to improve liver health, whereas the mechanism behind is still unknown. Herein, mice were gavaged with a strain of L. reuteri named SLZX19-12, which was isolated from faeces of healthy Tibetan pigs in our lab to investigate its effects on liver function. The results demonstrated that dietary L. reuteri changed the constituents of bile acid pool, especially influenced on metabolism of ursodeoxycholic acid (UDCA). In addition, L. reuteri reduced content of cholestasis-associated microbiota and effectively prevented liver from inflammatory response and hepatocyte apoptosis under the acute stimulation from LPS. We further revealed UDCA played an important part in protection of liver health during the stimulation of inflammatory factors in vitro. This study provides theoretical support and novel mechanism of action of L. reuteri protects liver from injury and influences on host physical health via regulation of crosstalk between bile acids and gut microbiota.

Impact of water flow rate on finishing pig performance.

A survey of 23 South Dakota pork producers in 2019 reported that 68% of the waterers in finishing barns had water flow rates above the recommended rate of 500-1,000 mL/min. The objective of the two studies was to determine the impact of water flow rate on finishing pig performance in the summer months. Study 1 used a total of 396 pigs in two groups in a 77-day trial (35.0 to 104.3 kg BW) with 6 pigs/pen and 1 cup waterer/pen. Study 2, conducted in a commercial style barn, used a total of 1,227 pigs in an 84-day trial (60.9 to 117.4 kg BW) with 26 pigs/pen and 2 cup waters/pen. Pens were assigned to one of three water flow rates (high, medium, low) based on the 3-hole settings of the water nipples (2.0, 1.0, and 0.8 mm; n = 22 and 16 pens/treatment for Study 1 and 2, respectively). Room temperature, outside temperature and relative humidity were recorded daily for both studies. In Study 1, water disappearance was recorded daily, and individual pen water flow rates were recorded every two weeks. At every diet phase change (26 ±â€…2.6 days), feed disappearance and individual pig body weights were recorded. Water flow rates averaged 1856 ±â€…188, 906 ±â€…214, 508 ±â€…100 mL/min for high, medium, and low flow settings, respectively. In Study 2, individual pen water flow rate, water disappearance, BW, and feed disappearance were recorded every two weeks. Water flow rates averaged 1115 ±â€…98, 906 ±â€…209, and 605 ±â€…203 mL/min for high, medium, and low flow settings, respectively. In both studies, there were no differences in final BW, cumulative ADG, or G:F. Due to the variability of water flow rate within a setting, data was further analyzed using regression with flow rate as the independent variable. Apart from average daily water disappearance (adj. R 2 = 0.87), there was a low relationship between pig performance and water flow rate (adj. R 2 < 0.09). The low R 2 values associated with pig performance and the high association with water disappearance suggests that water flow rate above current recommendations has little impact on finishing pig performance but does contribute to water wastage and its associated costs.

A systematic review and meta-analysis of dietary fat effects on reproductive performance of sows and growth performance of piglets.

Effects of added fat in sow diets on the sows' reproductive performance and offspring growth performance are influenced by multiple factors such as genetics, nutrition, parity, ambient temperatures, and farm management. Individual studies cannot cover all these factors. With the view to address this challenge, we searched all studies that were published from 1986 to 2020, and performed a systematic review and meta-analysis on the fat effect. In total, 19 papers were collected and analyzed. Fat supplementation in sow diets during late gestation and lactation decreased average daily feed intake (ADFI, P < 0.05) and tended to increase average daily energy intake (ADEI, P = 0.11). It had no impact on litter weights at birth (P = 0.40) or weaning (P = 0.46). It increased total numbers of piglets at birth (P = 0.07), but had no effect on liveborn per litter (P = 0.90) or survival rate (P = 0.48) of piglets to weaning. Fat supplementation had no significant effect on sow body weight loss (P = 0.67) or backfat thickness changes (P = 0.66), but sows fed diets with added fat had increased milk fat concentration (P = 0.03) and shorter wean to estrus intervals (WEI, P = 0.01). In specific circumstances, fat supplementation tended to improve growth performance of piglets with low litter weights at birth (P = 0.14), or when the sows lost large amounts of body weight during lactation (P = 0.11). The level of supplemented fat was 10% and higher would decrease liveborn per litter at neutral temperature (P = 0.10). The meta-analysis revealed that fat supplementation to sows diet during late gestation and lactation can be beneficial for sow reproductive performance and litter growth performance.

Bioavailable Lysine Assessed Using the Indicator Amino Acid Oxidation Method in Healthy Young Males is High when Sorghum is Cooked by a Moist Cooking Method.

BACKGROUND: Sorghum is the fifth most consumed cereal grain but limiting in the indispensable amino acid lysine. Complementing sorghum with lentils can improve the quality of sorghum-based diets. However, knowledge of lysine bioavailability in sorghum is lacking. OBJECTIVES: The study objectives were to determine the bioavailability of lysine in sorghum and to assess the effect of complementation of sorghum and lentils in a mixed-meal format. METHODS: We studied 5 healthy young men (≤30 years; BMI <25 kg/m2) in a repeated-measure design using the indicator amino acid oxidation (IAAO) method, with L-[1-13C] phenylalanine as the indicator. Each subject participated in 8 determinations in random order. On the reference diet, subjects received 4 amounts of L-lysine (5, 8, 12, and 15 mg. kg-1 . d-1) from a crystalline amino acid mixture patterned after egg protein. On the test diet, they received 3 levels of lysine (8.2, 12.5, and 15.7 mg. kg-1 . d-1) from sorghum, and on the complementation diet they received 1 level of lysine from a mixed meal of sorghum and lentils. The bioavailability of lysine in sorghum was estimated by comparing the IAAO response to the test diet with the IAAO response to the reference diet using the slope-ratio method. Effectiveness of complementation was assessed by comparing the IAAO response to the mixed meal to the IAAO response to the test protein. RESULTS: The bioavailability of lysine from sorghum was 94%. Upon complementation with lentils, there was a decline in the oxidation of L-[1-13C] phenylalanine by 19% (P < 0.0495), reflecting an improvement in available lysine in the mixed meal due to increased lysine intake. CONCLUSIONS: Although the bioavailability of lysine in sorghum is high, its lysine content is limiting. Complementation with lentils in a 1:1 ratio is recommended to achieve the lysine requirement for adult men consuming a sorghum-based diet. This trial was registered at clinicaltrials.gov as NCT03411005.

Mint Oil, ɤ-Tocopherol, and Whole Yeast Cell in Sow Diets Enhance Offspring Performance in the Postweaning Period.

Times of high metabolic activity in gestation and lactation, as well as periods of stress at weaning, can lead to greater incidences of oxidative stress in the dam and offspring during the suckling and postweaning period. Oxidative stress is an imbalance between prooxidant molecules and the antioxidant defense system that can negatively impact growth and/or reproductive performance. The objective of this research was to evaluate the effectiveness of whole yeast cell, peppermint oil, and ɤ-tocopherol in gestation and lactation on maternal oxidative status and offspring growth from birth to market. In study 1, 45 sows and gilts were assigned to one of four diets [control diet (CON), control + whole yeast cell (YC), control + mint oil top dress (MO), and control + yeast cell and mint oil top dress (YCMO)] provided from d110 of gestation through to weaning. A total of 481 weaned offspring were randomly allotted to pens balanced by weight and litter within maternal treatment and received the same dietary treatment as the sow for 35 days postwean in a four-phase feeding regimen. In study 2, 53 sows and gilts were allotted to four diet regimens similar to study 1 [CON, YC, MO, and control + ɤ-tocopherol (GT)] from d5 postbreeding to weaning. At weaning, 605 piglets were randomly allotted to pens, balanced by weight and litter within maternal treatment and fed a common diet for 126 days postwean in a nine-phase feeding regimen. Maternal dietary treatment did not impact sow body weight, piglet birth weight, and litter size in either study. In study 1, piglets from YC sows were heavier (p < 0.05) at weaning than CON animals. In the postwean period, overall daily gain was greater (p < 0.05) for CON-fed pigs than YCMO pigs, with overall feed intake greater (p < 0.05) for YCMO- than MO-fed pigs, resulting in lower (p < 0.05) Gain to Feed (G:F) in YCMO-fed pigs. In study 1, glutathione content in milk tended to be lower (p < 0.10) in MO than in YCMO sows. In study 2, piglets from GT-fed sows tended to be heavier (p < 0.10) at weaning than YC piglets. Lightweight pigs from CON sows tended to be lighter (p < 0.10) than pigs from all other treatment groups at weaning and day (d) 29 postwean. Lightweight MO and GT pigs were heavier at d42 (p < 0.05) than CON and YC pigs. At d70 postwean, GT pigs tended to be heavier than CON pigs. Lightweight MO pigs had greater gain (p < 0.05) during the finishing period than all other treatment groups. With respect to sow oxidative status in study 2, glutathione content in colostrum and d4 and 14 milk samples did not differ by maternal treatment. Superoxide dismutase activity in sow sera, colostrum, and milk did not differ between diets in either study. Whole yeast cell and ɤ-tocopherol supplementation in sow lactation diets resulted in heavier offspring. However, pre- and postnatal exposure to mint oil benefited lightweight pigs up to market weight.

Efficiency of standardized ileal digestible lysine utilization for whole body protein deposition in pregnant gilts and sows during early-, mid-, and late-gestation.

The efficiency of SID Lys utilization (kSID Lys) in gilts and sows during early (days 48 to 52), mid (days 75 to 79), and late gestation (days 103 to 107) was investigated using 88 pregnant females (PIC 1050; 27 gilts, 27 parity 1 sows, 34 parity 2+ sows; 192.96 ± 22.84 kg at days 42 ±1 of gestation) and whole body nitrogen (N) retention balance studies. Females were assigned to 1 of 4 SID Lys levels ranging from 40% to 70% of the daily SID Lys requirements above maintenance for a parity 1 sow according to the NRC (2012) gestating sow model in each gestation period. Experimental diets were isocaloric (3,335 kcal ME/kg) and isoproteic (11.75 % CP) and dietary indispensable AA were set to meet or exceed 100% of AA:Lys ratios. The slope of the linear response to graded SID Lys intake was defined as kSID Lys. With the aim of increasing the accuracy of kSID Lys estimates, gilt data from the current study was combined with gilt data from a previous study conducted at the same facility using 4 SID Lys levels ranging from 60% to 90% of the daily SID Lys requirements above maintenance for gilts according to the NRC (2012) gestating sow model. Whole body Lys retention of the combined gilt data set was assessed with different broken-line and nonlinear models. The kSID Lys was 0.65, 0.38, and 0.52 for early-, mid-, and late-gestation, respectively, in gilts. A linear response to graded SID Lys intake was found in late gestation only in parity 1 and 2+ sows; kSID Lys was determined as 0.44 and 0.52 in late gestation for parity 1 and parity 2+ sows, respectively. There were no differences in kSID Lys in late gestation between parities. For the combined gilt data, the model of best performance (reduced error and greater goodness of fit) was the Hoerl model. Maximum kSID Lys (i.e., g SID Lys retention/g SID Lys intake) in gilts was 0.67, 0.54, and 0.53 in early, mid, and late gestation predicted at 7.2, 9.1, and 13.5 g of SID Lys intake/d, respectively, based on the Hoerl model. Maximum SID Lys retention in gilts was similarly predicted at 8.5, 10.5, and 20.9 g of SID Lys intake per day in early, mid, and late gestation and resultant kSID Lys of 0.61, 0.51, and 0.44, respectively. The findings of this study demonstrate that kSID Lys varies by stage of gestation and SID Lys intake level and that, at least in gilts, a dynamic kSID adjusted for daily intake more adequately reflects biological response and hence allows more precise feeding of pregnant females.

Bioavailable Lysine, Assessed in Healthy Young Men Using Indicator Amino Acid Oxidation, is Greater when Cooked Millet and Stewed Canadian Lentils are Combined.

BACKGROUND: Pearl millet is the chief source of energy in the diet in some developing regions, but has a limited amount of indispensable amino acid lysine. Complementation with pulses like lentils can improve the protein quality of millet diets, but the knowledge of lysine bioavailability (BA) in millet and lentils is lacking. OBJECTIVES: The study objectives were to determine the BA of lysine in millet and lentils separately and to assess the effect of complementation of millet and lentils in a mixed meal format. METHODS: We studied 9 healthy young men (≤30 y; BMI <25) in a repeated-measure design using the indicator amino acid oxidation (IAAO) method, with L-[1-13C] phenylalanine as the indicator. Each subject completed 7 or 8 experiments in random order. On the reference diet, subjects received 4 graded levels of L-lysine (5, 8, 12, and 15 mg·kg-1.d-1) from a crystalline amino acid mixture patterned after egg protein; on the test diets, they received 3 levels of lysine (10, 12, and 15 mg·kg-1.d-1) from either steamed millet or stewed lentils; and on the complementation diet, they received 1 level of lysine from a mixed meal of steamed millet and stewed lentils. The BA of lysine and the effect of complementation were assessed by comparing the IAAO responses to the test diets and the complementation diet with the IAAO response to L-lysine intakes in the reference protein, using the slope ratio method. RESULTS: The BA of lysine was 97% from millet and 80% from lentils. Complementation of steamed millet with stewed lentils decreased the oxidation of L-[1-13C] phenylalanine by 27% (P < 0.05), signifying improved quality of the combined millet and lentil protein. CONCLUSIONS: Lysine has high BA but is still limiting in steamed pearl millet. Complementation with lentils in a 2:1 ratio is recommended to meet the lysine and protein requirements for adult men consuming a millet-based diet. This trial was registered at clinicaltrials.gov as NCT03674736 and NCT03339167.

Transcriptome Differences Suggest Novel Mechanisms for Intrauterine Growth Restriction Mediated Dysfunction in Small Intestine of Neonatal Piglets.

Impaired intestinal function is frequently detected in newborns with intrauterine growth restriction (IUGR), whereas the mechanism between transcriptome profiles and small intestinal dysfunction is still unclear. Therefore, this study was conducted by using IUGR neonatal piglets to uncover the mechanism underlying intestinal dysfunction. Neonatal piglets with IUGR and normal birth weight (NBW) were sacrificed at birth. Transcriptomic sequencing was performed on jejunum samples and generated 18,997 and 17,531 genes in NBW and IUGR groups, respectively. A total of 10 differentially expressed genes (DEGs) were identified; of note, only seven were mapped to the genome reference database, with two up-regulated (HSF4 and NR1H4; heat shock transcription factor 4 and nuclear receptor subfamily 1 group H member 4, respectively) and five down-regulated (SLC35C1, BTNL3, BPI, NLRP6, and SLC5A8; Solute carrier family 35 member C1, butyrophilin like 3, bactericidal permeability increasing protein, NLR family pyrin domain containing 6, and solute carrier family 5 member 8, respectively). Combining an enrichment analysis and reverse transcriptase-quantitative polymerase chain reaction validation of DEGs, our results proved the lipid metabolism disorder, intestinal dysfunction, and inflammatory response in IUGR piglets. Here, IUGR piglets presented lower concentration of glucose and triglyceride and higher concentration of total cholesterol and low-density lipoprotein cholesterol in plasma, compared with NBW piglets. Histological analysis revealed decreased mucins and increased apoptosis in both jejunum and ileum for IUGR piglets. Collectively, we found that IUGR induced intestinal dysfunction by altering lipid metabolism, intestinal barrier, and inflammatory response in neonatal piglets at birth, which provides new insights into the prevention and treatment of IUGR that protects against metabolic disorders and inflammatory-related diseases.

Effects of dietary crude protein level and N-carbamylglutamate supplementation on nutrient digestibility and digestive enzyme activity of jejunum in growing pigs.

Three experiments were conducted to investigate the effects of dietary crude protein (CP) level and N-carbamylglutamate (NCG) supplementation on apparent total tract digestibility (ATTD) and ileal digestibility of nutrients and digestive enzyme activity of jejunum in growing pigs. In experiment 1, 10 Duroc × Landrace × Yorkshire barrows (initial BW: 48.7 kg) were allotted to a three-period switchback design with five experimental diets and two replicate pigs per diet in each period. Diets were categorized as high CP (HP, 18% CP), moderate low CP (MLP, 15% CP), very low CP (VLP, 12% CP), and MLP and VLP with 0.1% NCG supplementation. Feces and urine were collected from day 6 to day 11 after a 5-d adaptation period. The DE, ME, and ATTD of GE, OM, CP, NDF, ADF, and P decreased (P < 0.01) with a reduction of dietary CP, but no effect of dietary treatments on pig daily N retention was detected. The NCG supplementation increased (P < 0.01) DE and ATTD of ADF of the VLP diet. In experiment 2, 10 jejunal-cannulated Duroc × Landrace × Yorkshire barrows (initial BW: 44.5 kg) were fed five diets for three periods as experiment 1. Jejunal fluid was collected on days 6 and 8 after a 5-d adaptation period. The digestive enzymes activity was not affected by dietary CP level, except for α-amylase, for which there was a decrease (P < 0.01) in pigs fed VLP diets compared to HP and MLP diets. In experiment 3, 12 ileal-cannulated Duroc × Landrace × Yorkshire barrows (initial BW: 46.7 kg) were allotted to a three-period switchback design with six diets and two replicate pigs per diet in each period. The six experimental diets consisted of five experimental diets as experiment 1 and one N-free diet. Ileal digesta was collected from day 6 to day 8 after a 5-d adaptation period. Results indicated that apparent ileal digestibility (AID) of CP and P and ileal digestibility of Arg, His, Ile, Leu, Phe, and all dispensable AA, except Pro, decreased (P < 0.01) in pigs fed VLP diet compared to HP and MLP diets, but AID of GE, OM, EE, NDF, and ADF were not affected. The supplementation of NCG in the VLP diet increased (P < 0.01) the AID of CP and ileal digestibility of Arg, His, Leu, Phe, Val, Ser, and Tyr. In conclusion, reducing dietary CP level decreased nutrient digestibility, but improved the efficiency of dietary N utilization and reduced N emission. Moderate reduction of dietary CP level had a minimal effect on nutrient digestibility and digestive enzyme activity. Additionally, NCG supplementation plays a beneficial effect on nutrient digestion only if the dietary CP level is extremely lowered.

Dietary inclusion of Peptiva, a peptide-based feed additive, can accelerate the maturation of the fecal bacterial microbiome in weaned pigs.

BACKGROUND: Weaning is one of the most critical transition stages of the swine production cycle, as the piglet gut physiology and microbiome need to rapidly adapt to changes in diet and environmental conditions. Based on their potential for producing a vast array of bioactive molecules, peptide formulations represent a largely untapped source of compounds that could be developed into feed additives to benefit animal health and nutrition. In this context, a commercial-scale nursery trial was performed to evaluate the impact of low inclusion of a peptide-based feed additive (Peptiva, Vitech Bio-Chem Corporation) on the performance and fecal microbiome of weaned pigs. RESULTS: While no significant differences in body weight, daily gain, daily feed intake nor gain:feed were observed between control and treatment animals (P > 0.05), an effect of Peptiva on the fecal bacterial composition of weaned pigs was observed. The first main observation was that the fecal bacterial profiles from pigs fed Control-Phase II and Control Phase III diets were found to be very distinct, suggesting that a transition or succession stage had occurred between the two phases. Lactobacilli, represented by four main OTUs (Ssd-00002, Ssd-00019, Ssd-00025, and Ssd-00053), were more abundant at the end of Phase II (P < 0.05), while Streptococci, mostly represented by OTUs Ssd-00039 and Ssd-00048, were in higher abundance at the end of Phase III (P < 0.05). Secondly, the fecal bacterial composition from pigs fed Peptiva Phase II diets showed similarities to both Control-Phase II and Control Phase III samples, while there was no difference in fecal bacterial composition between Control-Phase III and Peptiva Phase III samples. For instance, OTUs Ssd-00019,and Ssd-00053 were in lower abundance in Peptiva Phase II samples compared to Control Phase II (P < 0.05), but no significant difference was observed in the abundance of these two OTUs when comparing Peptiva Phase II to Control Phase III (P > 0.05). CONCLUSIONS: Together, these results suggest that Peptiva can modulate the composition of the swine microbiome during a specific window of the nursery stage, potentially by accelerating its maturation.

Branched chain amino acids alter fatty acid profile in colostrum of sows fed a high fat diet.

BACKGROUND: Branched chain amino acids (BCAAs) are important substrates for milk protein synthesis in the mammary gland, and are tightly related to lipid metabolism. No study has been performed examining the role of BCAAs with high fat diets on milk fat synthesis. This study was designed to investigate the effect of dietary BCAAs on growth performance of piglets, progeny body weight, and milk fat composition in sows fed a high fat diet. Four diets (CON = control diet; HF = high fat diet with 8% soybean oil; HF-MB=HF plus 0.39% BCAAs; HF-HB=HF plus 0.78% BCAAs) were fed to sows from late gestation to weaning. RESULTS: Compared to HF, BCAAs (HF-MB and HF-HB) increased the litter weight (P < 0.05) and overall litter weight gain (P < 0.05) at weaning and increased colostrum fat content by 27.3-35.8% (P < 0.01). Fatty acid profiles between the two doses of BCAAs were similar. Compared with HF, HF-MB tended to decrease the percentage of C18:3n3 (P = 0.063) and increased the percentage of C18:1n9c (P = 0.03). In addition, BCAAs in HF-MB increased the concentration of total fatty acid by 22.1% in colostrum (P = 0.03) but decreased that in serum at parturition by 53.2% (P = 0.027). The fatty acids in colostrum that increased with BCAAs were C15:0, C17:0, C20:3n6, C20:4n6, C20:5n3 and C22:6n3 (P = 0.00~0.04). Colostrum fatty acids of C20:0, C21:0, C22:0, C16:1, C20:1, C18:1n9c also tended to be increased (0.05 < P < 0.1) with BCAAs. The change in sow serum fatty acid profile due to BCAAs was different from that in colostrum. CONCLUSIONS: BCAAs in high fat diet of sows altered the fatty acid composition in colostrum and enhanced litter growth. Our study indicated that BCAAs supplementation can enhance mammary fatty acid uptake and mammary fat synthesis and that supplemental BCAAs and fat in late gestation and lactation diets for sows can improve reproductive performance.

The effect of pregnancy on nitrogen retention, maternal insulin sensitivity, and mRNA abundance of genes involved in energy and amino acid metabolism in gilts.

Twenty-one of each pregnant (P) and nonserviced, nonpregnant (NP) sister-pairs of gilts were selected to investigate the effect of pregnancy on protein deposition (Pd; whole body and maternal), insulin sensitivity, and mRNA abundance of genes involved in energy and AA metabolism. Between breeding (study day 0) and day 111, P and NP gilts received 2.16 kg of the experimental diet (3.34 Mcal ME/kg, 17.6% crude protein, 0.78% standardized ileal digestible lysine) that was formulated to meet the estimated ME requirements of pregnant gilts (and meet or exceed AA requirements). Nitrogen balances were conducted on day 63 and 102 ± 0.2 of the study during 4-d periods. Blood samples were collected on day 43, 56, 71, 85, 98, and 108 ± 0.3 of the study to determine plasma concentrations of fasted IGF-1, estradiol (E2), and estrone sulfate (E1S). Frequently sampled intravenous glucose tolerance tests (FSIGTT) were conducted on day 75 ± 0.7 in 6 P and 5 NP gilts and on day 107 ± 0.4 in 17 P and 17 NP gilts and the MINMOD approach was applied to evaluate whole body insulin sensitivity and pancreatic responsiveness. Longissimus muscle (LM) and s.c. adipose tissue (AD) samples were excised from 12 P and 12 NP gilts at day 111 ± 0.4 of the study after euthanasia to determine mRNA abundance of key genes. Whole body Pd was greater (P < 0.001) at day 102 and maternal Pd was lower (P < 0.002) at day 63 and 102 for P compared to NP gilts. Plasma concentrations of E1S and E2 increased (P < 0.05) with study day for P gilts and remained constant for NP gilts, which coincided with reduced plasma concentrations of IGF-1 and increased estrogen receptor alpha (ESR1) mRNA abundance in LM of P gilts. Glucose effectiveness was not different between P and NP gilts, but whole body insulin sensitivity was lower (P = 0.004) in P compared to NP gilts on day 75 and 107, which corresponded with reduced mRNA abundances of SLC2A4, HK2, SREBF1, and FASN, and increased abundances of PDK4 and PPARGC1A in LM and AD. When fed identically, P gilts had greater whole body Pd at day 102, which reflects Pd in the pregnancy-associated tissues (at the expense of maternal Pd), likely driven by estrogen-stimulated insulin resistance in peripheral tissue and subsequent modulation of gene expression relating to glucose metabolism.